Porcine Malic Enzyme ELISA Kit

malic enzyme, isoform C

Principle of the Assay: ME ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ME antibody and an ME-HRP conjugate. The assay sample and buffer are incubated together with ME-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ME concentration since ME from samples and ME-HRP conjugate compete for the anti-ME antibody binding site. Since the number of sites is limited, as more sites are occupied by ME from the sample, fewer sites are left to bind ME-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ME concentration in each sample is interpolated from this standard curve.

Porcine

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

47173

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