Porcine Peroxisome Proliferator Activated Receptor Delta ELISA Kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated PPARdelta concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine PPARdelta antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PPARdelta and PPARdelta antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PPARdelta in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Porcine

31.2 pg/ml - 1000 pg/ml.

Sandwich

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

5.0 pg/ml.