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PPARgamma (81B8) Rabbit mAb
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PPARγ (81B8) Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His494 of human PPARγ.
Product Usage Information
For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:200 |
| Chromatin IP | 1:100 |
| Chromatin IP-seq | 1:100 |
Specificity/Sensitivity
Species Reactivity:
Human, Mouse
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
参考图片
Chromatin immunoprecipitations were performed with cross-linked chromatin from untreated HDLM-2 cells and PPARγ (81B8) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Library was prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 14 (upper), including STON2 (lower), a known target gene of PPARγ (see additional figure containing ChIP-qPCR data).
Western blot analysis of extracts from NIH/3T3 and 3T3-L1 cells (differentiated 6 days) using PPARγ (81B8) Rabbit mAb.
Immunoprecipitation of PPARγ from HDLM-2 cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PPARγ (81B8) Rabbit mAb #2443. Western blot was performed using PPARγ (81B8) Rabbit mAb #2443.
Confocal immunofluorescent analysis of 3T3-L1 cells, using PPARγ (81B8) Rabbit mAb (red) showing nuclear localization in differentiated cells. Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Chromatin immunoprecipitations were performed with cross-linked chromatin from HDLM-2 cells and either PPARγ (81B8) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human FOXN3 Intron3 Primers #95568, STON2 intron 4 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Chromatin immunoprecipitations were performed with cross-linked chromatin from untreated HDLM-2 cells and PPARγ (81B8) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Library was prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figure shows binding across STON2, a known target gene of PPARγ (see additional figure containing ChIP-qPCR data).