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alpha-Smooth Muscle Actin (D4K9N) XP ®  Rabbit mAb
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alpha-Smooth Muscle Actin (D4K9N) XP ® Rabbit mAb

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分子量:
42
反应种属:
Human,Mouse,Rat,
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产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
alpha-Smooth Muscle Actin
来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human α-smooth muscle actin protein.

宿主
Rabbit
商品描述

Product Usage Information

ApplicationDilution
Western Blotting1:1000
Simple Western™1:10 - 1:50
Immunoprecipitation1:100
IHC Leica Bond1:100 - 1:400
Immunohistochemistry (Paraffin)1:320 - 1:1280
Immunofluorescence (Frozen)1:200 - 1:800
同种型
Rabbit IgG
分子量
42
研究领域
癌症,细胞生物学,纤维化,神经科学,
应用
反应种属
Human,Mouse,Rat,
目标/特异性

Specificity/Sensitivity

α-Smooth Muscle Actin (D4K9N) XP Rabbit mAb recognizes endogenous levels of total α-smooth muscle protein.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey

敏感性
Endogenous
背景
背景
Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility (1). The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells (1). 1.Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55.
研究领域
癌症,细胞生物学,纤维化,神经科学,
翻译后修饰
unmodified
制备和贮存
保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #50513.

数据库链接
Entrez-Gene ID
59
UniProt ID
P62736

参考图片

Confocal immunofluorescent analysis of mouse small intestine (left) and skeletal muscle (right) using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western blotting analysis of extracts from various human, mouse, and rat tissues using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (upper) and GAPDH #5174 (lower). As expected, skeletal muscle samples are negative for α-smooth muscle actin.

Simple Western™ analysis of extracts (1 mg/mL) from Human Colon tissue using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb #19245. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Immunoprecipitation of α-smooth muscle actin from mouse colon tissue extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb. Western blot analysis was performed using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human endometrioid carcinoma using alpha-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb performed on the Leica® Bond™ Rx.

Immunohistochemical analysis of paraffin-embedded human appendix using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse small intestine using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded rhesus monkey kidney using alpha-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded rhesus monkey spleen using alpha-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded rat colon using alpha-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded rat brain using alpha-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded Syrian hamster ovary using alpha-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded Syrian hamster uterus using alpha-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.

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货号:
19245T
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