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Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human α-smooth muscle actin protein.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:100 |
| IHC Leica Bond | 1:100 - 1:400 |
| Immunohistochemistry (Paraffin) | 1:320 - 1:1280 |
| Immunofluorescence (Frozen) | 1:200 - 1:800 |
Specificity/Sensitivity
Species Reactivity:
Human, Mouse, Rat, Hamster, Monkey
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #50513.
参考图片
Confocal immunofluorescent analysis of mouse small intestine (left) and skeletal muscle (right) using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Western blotting analysis of extracts from various human, mouse, and rat tissues using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (upper) and GAPDH #5174 (lower). As expected, skeletal muscle samples are negative for α-smooth muscle actin.
Simple Western™ analysis of extracts (1 mg/mL) from Human Colon tissue using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb #19245. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Immunoprecipitation of α-smooth muscle actin from mouse colon tissue extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb. Western blot analysis was performed using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human endometrioid carcinoma using alpha-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb performed on the Leica® Bond™ Rx.
Immunohistochemical analysis of paraffin-embedded human appendix using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse small intestine using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle using α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded rhesus monkey kidney using alpha-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded rhesus monkey spleen using alpha-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded rat colon using alpha-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded rat brain using alpha-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded Syrian hamster ovary using alpha-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded Syrian hamster uterus using alpha-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb.