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CDK Antibody Sampler Kit
CDK Antibody Sampler Kit
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CDK Antibody Sampler Kit

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    产品介绍
    产品信息
    抗原名称
    CDK
    来源纯化
    Monoclonal antibody is produced by immunizing animals with a recombinant human cdc2 fusion protein, synthetic peptides corresponding to residues of human CDK2 and recombinant human CDK7, and residues near the carboxy terminus of human CDK4, human CDK6, and human CDK9 proteins.
    简单描述
    Antibody Sampler Kit for studying Mouse IgG in the research area.
    应用
    目标/特异性

    Specificity/Sensitivity

    Each antibody in the CDK Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other family members.

    背景
    背景
    Cyclin-dependent kinases (CDKs) are the core effectors of cell cycle progression. CDK activity is regulated through association with their cyclin partners and cyclin-dependent kinase inhibitors (CKIs) as well as by activating and inhibitory phosphorylation events. Inhibition is mediated by Wee1 and Myt1 kinases that target residues at the amino terminus of CDK1 (1,2). Dephosphorylation of these residues by cdc25 phosphatase leads to activation of CDK kinase activity (3). The CDK7/cyclinH complex is the ubiquitous mammalian CDK-activating kinase (CAK) that phosphorylates a conserved threonine residue in the T-loop domain of CDKs. The carboxy-terminal domain of RNA polymerase II is also a target of CAK as well as CDK9/cyclinT (4,5). CDK4/6 associate with cyclinD and phosphorylate retinoblastoma protein and initiate progression through the restriction point in G1 (6). CDK2 associates with cyclinE in early S phase and cyclinA later in G2. CDK1/cyclinB regulates the initiation of mitotic events (7). 1.Fattaey, A. and Booher, R.N. (1997) Prog Cell Cycle Res 3, 233-40. 2.Booher, R.N. et al. (1997) J Biol Chem 272, 22300-6. 3.Jessus, C. and Ozon, R. (1995) Prog Cell Cycle Res 1, 215-28. 4.Kaldis, P. (1999) Cell Mol Life Sci 55, 284-96. 5.De Falco, G. and Giordano, A. Cancer Biol Ther 1, 342-7. 6.Sherr, C.J. (1995) Trends Biochem Sci 20, 187-90. 7.Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91.
    翻译后修饰
    Unmodified

    参考图片

    Flow cytometric analysis of Jurkat cells using CDK4 (D9G3E) Rabbit mAb and Propidium Iodid (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

    Western blot analysis of extracts from HeLa cells synchronized at various stages of the cell cycle, using cdc2 (POH1) Mouse mAb.

    After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

    After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

    Western blot analysis of extracts from SK-N-MC and C6 cells, using CDK7 (MO1) Mouse mAb.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using CDK7 (MO1) Mouse mAb.

    Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using CDK2 (78B2) Rabbit mAb.

    Flow cytometric analysis of Jurkat cells, using CDK2 (78B2) Rabbit mAb versus propidium iodide (DNA content).

    Western blot analysis of extracts from various cell types using CDK9 (C12F7) Rabbit mAb.

    Immunoprecipitation of CDK9 from HeLa cells using CDK9 (C12F7) Rabbit mAb. Western blot detection was performed using the same antibody. Lane 1 is 5% input.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CDK9 (C12F7) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).

    Flow cytometric analysis of Jurkat cells using CDK9 (C12F7) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

    Confocal immunofluorescent analysis of HeLa cells using CDK9 (C12F7) Rabbit mAb (green). Actin filaments have been labeled with DY-555 phalloidin (red).

    Immunohistochemical analysis of paraffin-embedded K7M2 mouse syngeneic tumor using CDK9 (C12F7) Rabbit mAb.

    Immunohistochemical analysis of frozen SKOV-3 xenograft using CDK9 (C12F7) Rabbit mAb.

    Western blot analysis of extracts from SK-N-MC and C6 cells using CDK7 (MO1) Mouse mAb #2916.Western blot方法检测SK-N-MC和 C6细胞提取物，使用的抗体为 CDK7 (MO1) Mouse mAb #2916。

    Western blot analysis of extracts from various cell types using CDK2 (78B2) Rabbit mAb #2546.Western blot 检测不同细胞提取物，使用的抗体为CDK2 (78B2) Rabbit mAb #2546。

    Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells using CDK9 (C12F7) Rabbit mAb #2316.Western blot方法检测HeLa, NIH/3T3 和C6 细胞提取物，使用的抗体为CDK9 (C12F7) Rabbit mAb #2316。

    Confocal immunofluorescent analysis of HT-29 cells, transfected with SignalSilence® Control siRNA (Unconjugated) #6568 (left), SignalSilence® cdc2 siRNA I #3500 (center) or SignalSilence® cdc2 siRNA II #3600 (right), using cdc2 (POH1) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    Western blot analysis of extracts from HeLa, Jurkat, and COS-7 cells using CDK6 (D4S8S) Rabbit mAb #13331.

    Western blot analysis of extracts from various cell lines using CDK4 (D9G3E) Rabbit mAb #12790.

    Western blot analysis of extracts from HeLa cells synchronized at various stages of the cell cycle, using cdc2 (POH1) Mouse mAb #9116.

    Confocal immunofluorescent analysis of MCF7 cells using CDK4 (D9G3E) Rabbit mAb (green), p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8493 (red), and Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #3458 (blue pseudocolor).

    Western blot analysis of extracts from various cell lines using CDK4 (D9G3E) Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CDK4 (D9G3E) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CDK4 (D9G3E) Rabbit mAb.

    Western blot analysis of extracts from HeLa, Jurkat, and COS-7 cells using CDK6 (D4S8S) Rabbit mAb.

    Immunoprecipitation of CDK6 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CDK6 (D4S8S) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using CDK6 (DCS83) Mouse mAb #3136.

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    货号：
    9868T
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