Bovine beta2-Glycoprotein ELISA Kit

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated ?2-GP concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine ?2-GP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ?2-GP and ?2-GP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ?2-GP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Bovine

0.5 ng/ml – 16 ng/ml.

Sandwich

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

0.1 ng/ml.