Fish Estrogen Receptor ELISA Kit

estrogen receptor

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated ER concentrations in Fish serum, plasma and other body fluids. Using Purified Fish ER antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ER and ER antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ER in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Fish

15.6 pg/ml – 500 pg/ml.

Sandwich

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

2.0 pg/ml.

2099

P03372 [Other Products]