Human Aprotinin ELISA Kit

aprotinin

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated AP concentrations in Human serum, plasma and other body fluids. Using Purified Human AP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AP and AP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human

62.5 pg/ml – 2000 pg/ml.

Sandwich

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

10 pg/ml.