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Human Protein Disulfide Isomerase A5 (PDIA5) ELISA Kit
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产品介绍
产品介绍
产品信息
抗原名称
protein disulfide-isomerase A5
商品描述
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDIA5 in human tissue homogenates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDIA5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PDIA5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDIA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PDIA5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
应用
反应种属
Human
检测范围
0.156-10ng/mL
检测类型
Sandwich
样本类型以及上样量
Tissue homogenates and other biological fluids.
敏感性
0.064ng/mL
背景
别名
PDI-A5; PDIR; Protein Disulfide Isomerase-Associated 5; Protein disulfide isomerase-related protein
制备和贮存
保存方式
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
数据库链接
NCBI
10954
UniProt ID
Q14554
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