全部商品分类首页
Mouse Angiotensin II (AngII) ELISA Kit
用小程序，查商品更便捷
产品介绍
产品介绍
产品信息
商品描述
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of AngII in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AngII. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AngII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AngII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AngII in the samples is then determined by comparing the O.D. of the samples to the standard curve.
应用
反应种属
Mouse
检测范围
31.2-2000pg/mL
检测类型
Quantitative Sandwich
样本类型以及上样量
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
敏感性
< 9.16pg/mL
背景
别名
Ang-II; Angiotensin-2
制备和贮存
保存方式
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。