全部商品分类首页
Porcine Haptoglobin (Hpt) ELISA Kit
用小程序，查商品更便捷
产品介绍
产品介绍
产品信息
抗原名称
haptoglobin
商品描述
Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Hpt in porcine serum, plasma and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hpt. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Hpt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hpt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hpt in the samples is then determined by comparing the O.D. of the samples to the standard curve.
应用
反应种属
Porcine
检测范围
78.125-5000ng/mL
检测类型
Sandwich
样本类型以及上样量
Serum, plasma and other biological fluids.
敏感性
25.5ng/mL
背景
别名
HP; Hp2-Alpha; Alpha-2-Macroglobulin; Zonulin
制备和贮存
保存方式
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
数据库链接
NCBI
397061
UniProt ID
Q8SPS7
[Other Products]
声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。