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Bovine Cathepsin D (CTSD) ELISA Kit
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产品介绍
产品介绍
产品信息
抗原名称
cathepsin D isoform X2
商品描述
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of CTSD in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to CTSD has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled CTSD and unlabeled CTSD (Standards or samples) with the pre-coated antibody specific to CTSD. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of CTSD in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of CTSD in the sample.
应用
反应种属
Bovine
检测范围
3.125-200ng/mL
检测类型
Competitive inhibition
样本类型以及上样量
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
敏感性
0.88ng/mL
背景
别名
CPSD; CLN10; Lysosomal Aspartyl Protease; Ceroid-Lipofuscinosis,Neuronal 10
制备和贮存
保存方式
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
数据库链接
NCBI
282883
UniProt ID
P80209
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