Monkey Adenylate Cyclase 4 ELISA Kit

ADCY4 protein, partial

Principle of the assay: ADCY4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADCY4 antibody and an ADCY4-HRP conjugate. The assay sample and buffer are incubated together with ADCY4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADCY4 concentration since ADCY4 from samples and ADCY4-HRP conjugate compete for the anti-ADCY4 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADCY4 from the sample, fewer sites are left to bind ADCY4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADCY4 concentration in each sample is interpolated from this standard curve.

Monkey

1.0-25ng/mL

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

0.1ng/mL

196883

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