Monkey Granulocyte Colony Stimulating Factor Receptor ELISA Kit

granulocyte colony-stimulating factor receptor isoform d

Principle of the assay: GCSFR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GCSFR antibody and an GCSFR-HRP conjugate. The assay sample and buffer are incubated together with GCSFR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCSFR concentration since GCSFR from samples and GCSFR-HRP conjugate compete for the anti-GCSFR antibody binding site. Since the number of sites is limited, as more sites are occupied by GCSFR from the sample, fewer sites are left to bind GCSFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCSFR concentration in each sample is interpolated from this standard curve.

Monkey

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

0.1 ng/mL.

1441

Q99062 [Other Products]