Guinea pig Cytochrome c oxidase ELISA Kit

cytochrome C oxidase

Principle of the assay: COX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COX antibody and an COX-HRP conjugate. The assay sample and buffer are incubated together with COX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COX concentration since COX from samples and COX-HRP conjugate compete for the anti-COX antibody binding site. Since the number of sites is limited, as more sites are occupied by COX from the sample, fewer sites are left to bind COX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COX concentration in each sample is interpolated from this standard curve.

Guinea Pig

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

0.1 ng/mL.