Bovine Kruppel Like Factor 18 ELISA Kit

Kruppel-like factor 18

Principle of the assay: KLF18 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KLF18 antibody and an KLF18-HRP conjugate. The assay sample and buffer are incubated together with KLF18-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLF18 concentration since KLF18 from samples and KLF18-HRP conjugate compete for the anti-KLF18 antibody binding site. Since the number of sites is limited, as more sites are occupied by KLF18 from the sample, fewer sites are left to bind KLF18-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLF18 concentration in each sample is interpolated from this standard curve.

Bovine

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

0.1 ng/mL.