Monkey Glutamate Receptor, Ionotropic, Delta 2 ELISA Kit

glutamate receptor ionotropic, delta-2 isoform 1

Intended Uses: This GRID2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey GRID2. This ELISA kit for research use only! Principle of the Assay: GRID2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRID2 antibody and an GRID2-HRP conjugate. The assay sample and buffer are incubated together with GRID2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRID2 concentration since GRID2 from samples and GRID2-HRP conjugate compete for the anti-GRID2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GRID2 from the sample, fewer sites are left to bind GRID2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRID2 concentration in each sample is interpolated from this standard curve.

Monkey

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

1.0 pg/mL.

2895

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