Canine Optineurin ELISA Kit

optineurin

Intended Uses: This OPTN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine OPTN. This ELISA kit for research use only! Principle of the Assay: OPTN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OPTN antibody and an OPTN-HRP conjugate. The assay sample and buffer are incubated together with OPTN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OPTN concentration since OPTN from samples and OPTN-HRP conjugate compete for the anti-OPTN antibody binding site. Since the number of sites is limited, as more sites are occupied by OPTN from the sample, fewer sites are left to bind OPTN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OPTN concentration in each sample is interpolated from this standard curve.

Canine

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

0.1 ng/mL.

10133

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