Bovine Vitellogenin ELISA Kit

vitellogenin

Intended Uses: This VTG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine VTG. This ELISA kit for research use only! Principle of the Assay: VTG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VTG antibody and an VTG-HRP conjugate. The assay sample and buffer are incubated together with VTG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VTG concentration since VTG from samples and VTG-HRP conjugate compete for the anti-VTG antibody binding site. Since the number of sites is limited, as more sites are occupied by VTG from the sample, fewer sites are left to bind VTG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VTG concentration in each sample is interpolated from this standard curve.

Bovine

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

1.0 ng/mL.