Human Intact Fibroblast Growth Factor 23 ELISA Kit

FGF23

Intended Uses: This iFGF-23 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human iFGF-23. This ELISA kit for research use only! Principle of the Assay: iFGF-23 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-iFGF-23 antibody and an iFGF-23-HRP conjugate. The assay sample and buffer are incubated together with iFGF-23-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the iFGF-23 concentration since iFGF-23 from samples and iFGF-23-HRP conjugate compete for the anti-iFGF-23 antibody binding site. Since the number of sites is limited, as more sites are occupied by iFGF-23 from the sample, fewer sites are left to bind iFGF-23-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The iFGF-23 concentration in each sample is interpolated from this standard curve.

Human

Quantitative Competitive

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

1.0 pg/mL

8074

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