Human Sulfatide antibody ELISA Kit

Intended Uses: This SAAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SAAb. Principle of the Assay: SAAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Sulfatide antigen and an SAAb-HRP conjugate. The assay sample and buffer are incubated together with SAAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SAAb concentration since SAAb from samples and SAAb-HRP conjugate compete for the Sulfatide antigen binding site. Since the number of sites is limited, as more sites are occupied by SAAb from the sample, fewer sites are left to bind SAAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SAAb concentration in each sample is interpolated from this standard curve.

Human

5.0-100ng/mL

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

1.0ng/mL