Human Neuregulin 1 Alpha ELISA Kit

neuregulin 1 alpha isoform, partial

Intended Uses: This NRG-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse NRG-1. This ELISA kit for research use only! Principle of the Assay: NRG-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NRG-1 antibody and an NRG-1-HRP conjugate. The assay sample and buffer are incubated together with NRG-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NRG-1 concentration since NRG-1 from samples and NRG-1-HRP conjugate compete for the anti-NRG-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by NRG-1 from the sample, fewer sites are left to bind NRG-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NRG-1 concentration in each sample is interpolated from this standard curve.

Human

Quantitative Competitive

Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate

1.0 ng/mL.