Guinea pig Anti-M2A IgG2a Antibody ELISA Kit

Intended Uses: This Anti-M2A IgG2a Antibody ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig Anti-M2A IgG2a Antibody. This ELISA kit for research use only! Principle of the Assay: Anti-M2A IgG2a Antibody ELISA kit applies the competitive enzyme immunoassay technique utilizing M2A IgG2a antigen and an Anti-M2A IgG2a Antibody-HRP conjugate. The assay sample and buffer are incubated together with Anti-M2A IgG2a Antibody-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-M2A IgG2a Antibody concentration since Anti-M2A IgG2a Antibody from samples and Anti-M2A IgG2a Antibody-HRP conjugate compete for the M2A IgG2a antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-M2A IgG2a Antibody from the sample, fewer sites are left to bind Anti-M2A IgG2a Antibody-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-M2A IgG2a Antibody concentration in each sample is interpolated from this standard curve.

Guinea Pig

5-100ng/mL

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

1.0ng/mL