Human Hedgehog Homolog, Indian ELISA Kit

Intended Uses: This IHH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IHH. This ELISA kit for research use only! Principle of the Assay: IHH ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IHH antibody and an IHH-HRP conjugate. The assay sample and buffer are incubated together with IHH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IHH concentration since IHH from samples and IHH-HRP conjugate compete for the anti-IHH antibody binding site. Since the number of sites is limited, as more sites are occupied by IHH from the sample, fewer sites are left to bind IHH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IHH concentration in each sample is interpolated from this standard curve.

Human

Quantitative Competitive

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

0.1 ng/mL.