Porcine RhoA Protein ELISA Kit

rhoA protein

Principle of the Assay: RhoA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RhoA antibody and an RhoA-HRP conjugate. The assay sample and buffer are incubated together with RhoA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RhoA concentration since RhoA from samples and RhoA-HRP conjugate compete for the anti-RhoA antibody binding site. Since the number of sites is limited, as more sites are occupied by RhoA from the sample, fewer sites are left to bind RhoA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RhoA concentration in each sample is interpolated from this standard curve.

Porcine

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate