Sheep Metallothionein-3 ELISA Kit

metallothionein-3

Principle of the Assay: MT-3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MT-3 antibody and an MT-3-HRP conjugate. The assay sample and buffer are incubated together with MT-3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MT-3 concentration since MT-3 from samples and MT-3-HRP conjugate compete for the anti-MT-3 antibody binding site. Since the number of sites is limited, as more sites are occupied by MT-3 from the sample, fewer sites are left to bind MT-3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MT-3 concentration in each sample is interpolated from this standard curve.

Sheep

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

4504

P25713 [Other Products]