Chicken N-Acetyl-Ser-Asp-Lys-Pro ELISA Kit

Principle of the Assay: AcSDKP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AcSDKP antibody and an AcSDKP-HRP conjugate. The assay sample and buffer are incubated together with AcSDKP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AcSDKP concentration since AcSDKP from samples and AcSDKP-HRP conjugate compete for the anti-AcSDKP antibody binding site. Since the number of sites is limited, as more sites are occupied by AcSDKP from the sample, fewer sites are left to bind AcSDKP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AcSDKP concentration in each sample is interpolated from this standard curve.

Chicken

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate