Canine Myeloid-related protein ELISA Kit

Mrp, partial

Principle of the Assay: MRP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MRP antibody and an MRP-HRP conjugate. The assay sample and buffer are incubated together with MRP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MRP concentration since MRP from samples and MRP-HRP conjugate compete for the anti-MRP antibody binding site. Since the number of sites is limited, as more sites are occupied by MRP from the sample, fewer sites are left to bind MRP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MRP concentration in each sample is interpolated from this standard curve.

Canine

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate