Guinea pig Protease Activated Receptor 4 ELISA Kit

proteinase-activated receptor 4

Principle of the Assay: PAR4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PAR4 antibody and an PAR4-HRP conjugate. The assay sample and buffer are incubated together with PAR4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAR4 concentration since PAR4 from samples and PAR4-HRP conjugate compete for the anti-PAR4 antibody binding site. Since the number of sites is limited, as more sites are occupied by PAR4 from the sample, fewer sites are left to bind PAR4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAR4 concentration in each sample is interpolated from this standard curve.

Guinea Pig

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

9002

Q96RI0 [Other Products]