Chicken Chromodomain Helicase DNA Binding Protein 3 ELISA Kit

chromodomain-helicase-DNA-binding protein 3 isoform 3

Principle of the Assay: CHD3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CHD3 antibody and an CHD3-HRP conjugate. The assay sample and buffer are incubated together with CHD3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHD3 concentration since CHD3 from samples and CHD3-HRP conjugate compete for the anti-CHD3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CHD3 from the sample, fewer sites are left to bind CHD3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHD3 concentration in each sample is interpolated from this standard curve.

Chicken

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

1107

Q12873 [Other Products]