Bovine Connexin 31 ELISA Kit

connexin 31

Principle of the Assay: CX31 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CX31 antibody and an CX31-HRP conjugate. The assay sample and buffer are incubated together with CX31-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CX31 concentration since CX31 from samples and CX31-HRP conjugate compete for the anti-CX31 antibody binding site. Since the number of sites is limited, as more sites are occupied by CX31 from the sample, fewer sites are left to bind CX31-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CX31 concentration in each sample is interpolated from this standard curve.

Bovine

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

2707

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