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Bovine Gap Junction protein, alpha 5, 40kd ELISA Kit

产品介绍
产品介绍
产品信息
商品描述
Principle of the Assay: GJA5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GJA5 antibody and an GJA5-HRP conjugate. The assay sample and buffer are incubated together with GJA5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GJA5 concentration since GJA5 from samples and GJA5-HRP conjugate compete for the anti-GJA5 antibody binding site. Since the number of sites is limited, as more sites are occupied by GJA5 from the sample, fewer sites are left to bind GJA5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GJA5 concentration in each sample is interpolated from this standard curve.

应用
反应种属
Bovine

检测类型
Competitive

样本类型以及上样量
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

制备和贮存
保存方式
Store all reagents at 2-8 degree C.
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