Mouse Anti-Ganglioside Q1b Antibody ELISA Kit

Intended Uses: This anti-GQ1b ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse anti-GQ1b. This ELISA kit for research use only! Principle of the Assay: Anti-GQ1b ELISA kit applies the competitive enzyme immunoassay technique utilizing a GQ1b antigen and an anti-GQ1b-HRP conjugate. The assay sample and buffer are incubated together with anti-GQ1b-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the anti-GQ1b concentration since anti-GQ1b from samples and anti-GQ1b-HRP conjugate compete for the GQ1b antigen binding site. Since the number of sites is limited, as more sites are occupied by anti-GQ1b from the sample, fewer sites are left to bind anti-GQ1b-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The anti-GQ1b concentration in each sample is interpolated from this standard curve.

Mouse

10-250ng/mL

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

1.0ng/mL