Bovine Anti-trypsin ELISA Kit

trypsin

Principle of the Assay: Trypsin-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Trypsin antigen and an Trypsin-Ab-HRP conjugate. The assay sample and buffer are incubated together with Trypsin-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Trypsin-Ab concentration since Trypsin-Ab from samples and Trypsin-Ab-HRP conjugate compete for the Trypsin antigen binding site. Since the number of sites is limited, as more sites are occupied by Trypsin-Ab from the sample, fewer sites are left to bind Trypsin-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Trypsin-Ab concentration in each sample is interpolated from this standard curve.

Bovine

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate