Human Sterile Alpha Motif Domain Containing 9 Like Protein ELISA Kit

sterile alpha motif domain-containing protein 9-like

Intended Uses: This SAMD9L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SAMD9L. This ELISA kit for research use only! Principle of the Assay: SAMD9L ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SAMD9L antibody and an SAMD9L-HRP conjugate. The assay sample and buffer are incubated together with SAMD9L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SAMD9L concentration since SAMD9L from samples and SAMD9L-HRP conjugate compete for the anti-SAMD9L antibody binding site. Since the number of sites is limited, as more sites are occupied by SAMD9L from the sample, fewer sites are left to bind SAMD9L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SAMD9L concentration in each sample is interpolated from this standard curve.

Human

5.0-100ng/mL

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

1.0ng/mL

219285

Q8IVG5 [Other Products]