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Canine Adenylyl Cyclase Associated Protein 1 ELISA Kit

产品介绍
产品介绍
产品信息
抗原名称
adenylyl cyclase-associated protein 1

商品描述
Intended Uses: This CAP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CAP1. This ELISA kit for research use only!
Principle of the Assay: CAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CAP1 antibody and an CAP1-HRP conjugate. The assay sample and buffer are incubated together with CAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAP1 concentration since CAP1 from samples and CAP1-HRP conjugate compete for the anti-CAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CAP1 from the sample, fewer sites are left to bind CAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAP1 concentration in each sample is interpolated from this standard curve.

应用
反应种属
Canine

检测范围
50-1000ng/mL

检测类型
Competitive

样本类型以及上样量
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

敏感性
1.0ng/mL

制备和贮存
保存方式
Store all reagents at 2-8 degree C.
数据库链接
NCBI
10487

UniProt ID
Q01518
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