Canine Adenylyl Cyclase Associated Protein 1 ELISA Kit

adenylyl cyclase-associated protein 1

Intended Uses: This CAP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CAP1. This ELISA kit for research use only! Principle of the Assay: CAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CAP1 antibody and an CAP1-HRP conjugate. The assay sample and buffer are incubated together with CAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAP1 concentration since CAP1 from samples and CAP1-HRP conjugate compete for the anti-CAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CAP1 from the sample, fewer sites are left to bind CAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAP1 concentration in each sample is interpolated from this standard curve.

Canine

50-1000ng/mL

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

1.0ng/mL

10487

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