ATG14 Recombinant Rabbit mAb (S-2371-15)

ATG14, also known as Barkor (Beclin 1-associated autophagy-related key regulator), is a 492-amino-acid protein essential for autophagy initiation in eukaryotes. It assembles with Beclin 1, VPS15, and the class III phosphatidylinositol 3-kinase VPS34 to form the PI3K complex I that nucleates isolation membranes and omegasomes, thereby triggering autophagosome biogenesis. ATG14 recognizes membrane curvature via its N-terminal cysteine-rich domain, which binds phosphatidylinositol 3-phosphate to tether pre-autophagosomal membranes, and subsequently promotes STX17-SNAP29 t-SNARE complex assembly to drive autophagosome–lysosome fusion [^3][^8]. Beyond canonical autophagy, ATG14 safeguards mitochondrial integrity and restrains gasdermin-mediated pyroptosis, enabling physiological processes such as hepatic lipid homeostasis and early-pregnancy embryo transport.