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THUNDER™ cAMP TR-FRET Assay Kit
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THUNDER™ cAMP TR-FRET Assay Kit
品牌： Bioauxilium
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咨询反应种属：
All species expected
来源宿主：
鼠
产品介绍
产品介绍
产品信息
抗原名称
cAMP
宿主
鼠
商品描述
Homogeneous competitive TR-FRET immunoassay for quantitative measurement of cAMP in cell lysates. This is a robust, sensitive, and easy-to-use assay for monitoring GPCR activation based on cAMP production in cells. The kit is compatible with both suspension and adherent cells and is suitable for the profiling and high-throughput screening of Gαs- and Gαi-coupled GPCRs. Detailed assay protocols for testing agonists and antagonists, as well as full assay validation can be found in the Technical Data Sheet. BEST SELLER
Components
cAMP standard (100 µL)
Biotin-cAMP (25 µL)
Eu-streptavidin (25 µL)
Acc-anti-cAMP (50 µL)
5X cAMP Detection Buffer (2 x 1 mL)
Reactivity
All species expected
Assay principle: Free cAMP produced by cells competes with the Europium labeled streptavidin/biotin-cAMP tracer complex for binding to a monoclonal cAMP antibody labeled with the far-red fluorophore. The specific TR-FRET signal is therefore inversely proportional to the concentration of cAMP in the lysate.
The THUNDER™ cAMP assay exhibits a comparable performance to that of a competing TR-FRET assay.
The THUNDER™ cAMP assay workflow consists of only two steps.
应用
反应种属
All species expected
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