Product Description

Applications

Detect endogenous acetylation changes on a protein of interest (POI) in cells or tissue extracts
Isolate the total acetylation profile from cells or tissues via immunoprecipitation (IP) for downstream proteomic analysis
Confirm POI acetylation data generated from mass spec proteomics

Material

Cytoskeleton offers two Acetyl-Lysine Affinity beads: AAC02-beads and AAC03-beads. Both contain mouse anti-acetyl-lysine antibodies covalently linked to protein G beads and enrich a broad range of acetylated proteins. Used together, they provide a more comprehensive enrichment profile.

While their specificities overlap, each has unique strengths—AAC02-beads may outperform AAC03-beads for specific targets. For a new protein of interest, test both separately to determine which gives the best results (see Detailed Methods and Validated Applications for examples).

AAC04-beads is a combo of Cat # AAC02-beads and Cat # AAC03-beads .

Key characteristics

2 tubes of each affinity bead AAC02-beads & AAC03-beads
Broad enrichment of acetylated proteins
No heavy/light chain leaching during IP
Complimentary overlap with AAC02-beads AAC02-beads - see datasheet for details
Sufficient reagent for 40 IP assays (1 mg lysate per IP)
Consistent lot-to-lot performance
Supplied as a lyophilized powder
Buffer composition after rehydration: 200 mM PIPES, 50% glycerol, 5% sucrose, and 1% dextran

Biological Activity Assay

AAC04-beads contain 2 separate tubes, each of AAC02/AAC03-beads, allowing the end-user the versatility to examine both affinity beads and a mix of the two to determine which conditions are optimal for their application.

Acetyl-Lysine Affinity Beads - AAC04