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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb (S-602-276)
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PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
ELISA
Sandwich ELISA
CLIA
Lateral Flow
Dot Blot
1:1000WB
1:1000IP
1:50IHC-P
ICC
IF
ICFCM
FCM
mIHC
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) refers to the phosphorylated form of the mitogen-activated protein kinases, also known as extracellular signal-regulated kinases 1 and 2. These kinases are part of the MAPK signaling pathway, which is a series of protein kinases that play a crucial role in regulating various cellular processes such as cell growth, differentiation, proliferation, and apoptosis. The phosphorylation of Erk1/2 at specific residues, Thr202 and Tyr204, is a key regulatory event that activates these kinases. When Erk1/2 are phosphorylated at these sites, they become active and can then phosphorylate a variety of target proteins, thereby transmitting signals from the cell membrane to the nucleus.
12 months from date of receipt / reconstitution, -20 °C as supplied
参考图片
Dot blot result of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb
Lane 1: p44/42 MAPK (Erk1/2) (Thr202/Tyr204) phospho peptide
Lane 2: p44/42 MAPK (Erk1/2) (Thr202) phospho peptide
Lane 3: p44/42 MAPK (Erk1/2) (Thr204) phospho peptide
Lane 4: p44/42 MAPK (Erk1/2) unmodified peptide
Primary antibody: Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
WB result of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb
Primary antibody: Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Jurkat whole cell lysate 20 µg
Lane 2: Jurkat treated with 200 nM TPA for 20 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42, 44 kDa
Observed MW: 38, 40 kDa
WB result of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb
Primary antibody: Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 200 nM TPA for 20 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42, 44 kDa
Observed MW: 38, 40 kDa
WB result of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb
Primary antibody: Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 200 nM TPA for 20 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42, 44 kDa
Observed MW: 38, 40 kDa