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THUNDER™Phospho-RIPK1(S166)TR-FRETCellSignalingAssayKit
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产品介绍
产品介绍
产品信息
抗原名称
RIPK1
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-RIPK1 (S166) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Components
Eu-phospho-RIPK1 (25 µL)
Acc-phospho-RIPK1 (100 µL)
RIPK1 control lysate (200 µL)
5X Lysis Buffer 1 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)
Reactivity
Human
Data show that treatment of HT-29 cells with Necrostatin-1S inhibits phosphorylation of RIPK1 at S166 by TNF-alpha. in the presence of SM-164 and Z-VAD.
The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values. following 1/2 dilution.
应用
反应种属
Human
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