THUNDER™TotalRIPK1TR-FRETCellSignalingAssayKit

品牌： Bioauxilium

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参数

反应种属：

Human

产品介绍

产品信息

抗原名称

RIPK1

商品描述

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total RIPK1 (both phosphorylated and non-phosphorylated RIPK1) in cell lysates. This kit can be used to normalize data obtained with the Phospho-RIPK1 (S166) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Components
Eu-total-RIPK1 (250 µL)
Acc-total-RIPK1 (1 mL)
RIPK1 control lysate (2 x 200 µL)
5X Lysis Buffer 1 (50 mL)
10X TR-FRET Detection buffer (2.5 mL)
100X Phosphatase Inhibitor Cocktail (2.5 mL)

Reactivity
Human

Inhibition of Phospho-RIPK1 in HT-29 cells

Data show that treatment of HT-29 cells with Necrostatin-1S inhibits phosphorylation of RIPK1 at S166 by TNF-alpha (in the presence of SM-164 and Z-VAD) but does not have a major effect on the levels of total RIPK1.

HT-29 control lysate titration (QC Test)

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

应用

反应种属

Human

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货号：

KIT-RIPK1T-5000

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询价

5000points

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