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Pfu DNA Polymerase Ⅱ
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产品介绍
产品介绍
产品信息
商品描述
产品组成：
1. Storage Solution : 5 U/ul Pfu DNA Polymerase Ⅱ, 20 mM Tris-HCl, 0.1 mM EDTA, 0.1% Tween20, 0.1% Triton X100, 1 mM DTT, 100 mM KCl, 50%Glycerol、pH 8.2 @ 25°C
2. 10× Reaction Buffer: 200 mM Tris-HCl (pH 8.8) , 20 mM MgSO4, 100 mM KCl, 100 mM (NH4) 2SO4, 1% Triton X-100, 1 mg/mL nuclease-free BSA
分子量
90 kDa (Reducing)
纯度
＞95% by SDS-PAGE
外观
Liquid
标签
His Tag
应用
注意事项
It is critical to withhold Pfu DNA Polymerase until after the addition of dNTPs; otherwise, the proofreading activity of the polymerase may degrade the primers,resulting in nonspecific amplification and reduced product yield. Assemble on ice.
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