全部商品分类
用小程序，查商品更便捷
ELISA
Sandwich ELISA
CLIA
Lateral Flow
Dot Blot
1:1000WB
1:1000IP
IHC-P
1:100-1:200ICC
IF
ICFCM
FCM
mIHC
EGFR is a 170 kDa transmembrane receptor that induces tyrosine kinase activity affecting cell growth. EGFR mutations can occur in various parts of the gene. In NSCLC, the mutations occur almost exclusively in the exons encoding the kinase domain (exons 18 to 21). They most commonly include the in-frame deletion of exon 19 (del19) and L858R mutation in exon 21.Del19 constitutes 44–51%, and exon 21 L858R mutation constitutes 38–40%.
12 months from date of receipt / reconstitution, -20 °C as supplied
参考图片
Dot blot result of EGFR (L858R) Recombinant Rabbit mAb
Lane1: EGFR(L858R) mutated peptide
Lane2: EGFR(L858R) mutated peptide
Lane3: EGFR unmutated peptide
Primary antibody: EGFR (L858R) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
WB result of EGFR (L858R) Recombinant Rabbit mAb
Primary antibody: EGFR (L858R) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: HCC827 whole cell lysate 20 µg
Lane 5: H1975 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate; A431 whole cell lysate; A549 whole cell lysate; HCC827 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 134 kDa
Observed MW: 150 kDa
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma (Mutated case). Anti-EGFR (L858R) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung adenocarcinoma (un-mutated case). Anti-EGFR (L858R) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung. Anti-EGFR (L858R) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Expression of EGFR (L858R) in tumor tissue
Expression of EGFR (L858R) in human tissue