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FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) (S-589-8)

FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) (S-589-8)

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反应种属:
Species Independent
来源宿主:
Rabbit
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产品介绍
产品信息
荧光素标记
纯化方式
Protein A
抗原名称
FLAG Tag
宿主
Rabbit
免疫原
Synthetic Peptide
同种型
IgG
克隆号
S-589-8
浓度
2 mg/ml
性状
Liquid
缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
产品类型
Recombinant mAb
应用
实验应用
WB
反应种属
Species Independent
稀释度
  • ELISA

  • Sandwich ELISA

  • CLIA

  • Lateral Flow

  • Dot Blot

  • WB

    1:5000-1:10000
  • IP

  • IHC-P

  • ICC

  • IF

  • ICFCM

  • FCM

  • mIHC

背景
背景

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. FLAG-tag has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. FLAG-tag can also be used in the isolation of protein complexes with multiple subunits.

制备和贮存
保存方式

12 months from date of receipt / reconstitution, -20 °C as supplied

参考图片

WB result of FLAG Tag Recombinant Rabbit mAb (HRP Conjugate)
Lysate: 293T transfected with Histone H3-Flag-V5 whole cell lysate 20 µg
Primary antibody:
Lane 1: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Lane 4: FLAG Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa

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货号:
S0B1648-1ml
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