THUNDER™Phospho-Rb(S807/S811)TR-FRETCellSignalingAssayKit

THUNDER™Phospho-Rb(S807/S811)TR-FRETCellSignalingAssayKit

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品牌: Bioauxilium
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    反应种属:
    Human
    Human
    来源宿主:
    产品介绍
    产品信息
    抗原名称
    RB
    宿主
    商品描述

    Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-Rb (S807/S811) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

    组成成分
    磷酸化视网膜细胞瘤抑癌蛋白抗体(铕离子标记),磷酸化视网膜细胞瘤抑癌蛋白抗体(远红光受体标记),裂解缓冲液(聚乙二醇单辛基苯基水溶液),检测缓冲液(磷酸盐缓冲液),细胞裂解液(三(羟甲基)氨基甲烷水溶液),磷酸酶抑制剂混合物(N-(2,2,2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺,水)

    Components
    Eu-phospho-Rb-S807 (25 µL)
    Acc-phospho-Rb-S807 (100 µL)
    Rb control lysate (200 µL)
    5X Lysis Buffer 1 (5 mL)
    10X TR-FRET Detection buffer (250 µL)
    100X Phosphatase Inhibitor Cocktail (50 µL)

    Reactivity
    Human

    Inhibition of Phospho-Rb-S807 in HT-29 cells
    Data show that treatment of HT-29 cells with palbociclib inhibits phosphorylation of Rb at S807/S811.
    Z'-factor of Phospho-Rb (S807) in Jurkat cells
    The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.
    应用
    反应种属
    Human
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    货号:
    KIT-RBS807P-500
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