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THUNDER™Phospho-Rb(S807/S811)TR-FRETCellSignalingAssayKit

产品介绍
产品信息
抗原名称
RB

宿主
鼠

商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-Rb (S807/S811) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
组成成分
磷酸化视网膜细胞瘤抑癌蛋白抗体(铕离子标记),磷酸化视网膜细胞瘤抑癌蛋白抗体(远红光受体标记),裂解缓冲液(聚乙二醇单辛基苯基水溶液),检测缓冲液(磷酸盐缓冲液),细胞裂解液(三(羟甲基)氨基甲烷水溶液),磷酸酶抑制剂混合物(N-(2,2,2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺,水)

Components
Eu-phospho-Rb-S807 (125 µL)
Acc-phospho-Rb-S807 (500 µL)
Rb control lysate (200 µL)
5X Lysis Buffer 1 (25 mL)
10X TR-FRET Detection buffer (1.25 mL)
100X Phosphatase Inhibitor Cocktail (1.25 mL)
Reactivity
Human

Data show that treatment of HT-29 cells with palbociclib inhibits phosphorylation of Rb at S807/S811.

The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.
应用
反应种属
Human

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