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THUNDER™Phospho-STAT2(Y690)TR-FRETCellSignalingAssayKit
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产品介绍
产品介绍
产品信息
抗原名称
STAT2
宿主
鼠
商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT2 (Y690) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
组成成分
磷酸化信号转导和转录激活因子2抗体（铕离子标记），磷酸化信号转导和转录激活因子2抗体（远红光受体标记），裂解缓冲液（聚乙二醇单辛基苯基水溶液），检测缓冲液（磷酸盐缓冲液），细胞裂解液（三(羟甲基)氨基甲烷水溶液），磷酸酶抑制剂混合物（N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）
Components
Eu-phospho-STAT2 (25 µL)
Acc-phospho-STAT2 (10 µL)
STAT2 control lysate (200 µL)
5X Lysis Buffer 1 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human, Mouse
Data show that treatment of A431 cells with IFNα2b stimulates phosphorylation of STAT2 at Y690.
Data show that treatment of A431 cells with JAK Inhibitor 1 inhibits phosphorylation of STAT2 at Y690 by IFNα2b.
The Z’-factor value of 0.86 indicates that the assay is robust and suitable for HTS
Data show a linear relationship between lysate dilutions and TR-FRET ratio value.
应用
反应种属
Human, Mouse
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