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ELISA
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mIHC
Tumor necrosis factor α (TNF alpha) is an effective pro-inflammatory cytokine, which can kill and inhibit tumor cells. It is mainly produced by activated macrophages and can also be secreted by other types of cells, such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils and neuronal tissues. The members of TNF alpha family exert their cellular effect through two distinct surface receptors of the TNF receptor family, TNFR1and TNFR2. The pleiotropic biological effects of TNF can be attributed to its ability to simultaneously activate multiple signaling pathways in cells. TNF-induced NF-κB activation promotes the growth, invasion and metastasis of cancer cells.
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
参考图片
Standard curve
Example of Mouse TNF-α standard curve in Assay Diluent #1.
Protocol Diagram
Linearity
The concentrations of TNF-α were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: RAW264.7 mouse monocyte/macrophage cells stimulated with 5ug/ml LPS and 100ng/mL rmIL-10 for 7days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 13.19 ng/mL in stimulated RAW264.7 supernatant.