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mIHC
TNF alpha is produced by various types of cells including macrophages, monocytes, neutrophils, T cells, and NK-cells. TNF-alpha is critical for normal immune response, abnormal secretion TNF alpha activates synovial fibroblasts, keratinocytes, osteoclasts, induces rheumatoid arthritis, inflammatory bowel disease, psoriatic arthritis (PsA), and noninfectious uveitis (NIU). TNF alpha positively regulates endogenous TNF-α expression levels independently of Pgp efflux activity, induces IHF cells proliferation. TNF alpha in tissues may promote cancer growth, invasion, and metastasis. Besides, TNF alpha stimulates NF-κB pathway via TNFR2 and anti-TNF-α MAb significantly suppresses the tumor development in colitis-associated cancer (CAC) mouse. TNF alpha as a proneurogenic factor activates the SAPK/JNK pathway and can facilitate neuronal replacement and brain repair in response to brain injury.
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
参考图片
Standard curve
Example of Human TNF-α standard curve in Assay Diluent #1.
Linearity
The concentrations of TNF-α were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human PBMC cells stimulated with 10ng/ml PMA and 500ng/mL lonomycin Calcium Salt for 24 hours (25%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 13.99 ng/mL in stimulated human PBMC supernatant.
Protocol Diagram